› 1s35

X-ray diffraction
2.4Å resolution

Crystal Structure of Repeats 8 and 9 of Human Erythroid Spectrin

Released:
DOI: 10.2210/pdb1s35/pdb
PDB entry 1s35 coloured by chain, front view.
PDB entry 1s35 coloured by chain, side view.
PDB entry 1s35 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into the stability and flexibility of unusual erythroid spectrin repeats.
Kusunoki H, MacDonald RI, Mondragón A
Structure 12 645-56 (2004)
PMID: 15062087

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145745 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spectrin beta chain, erythrocytic Chain: A
Molecule details ›
Chain: A
Length: 214 amino acids
Theoretical weight: 24.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11277 (Residues: 1063-1275; Coverage: 10%)
Gene names: SPTB, SPTB1
Sequence domains: Spectrin repeat
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P41212
Unit cell:
a: 122.151Å b: 122.151Å c: 49.548Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 0.259
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Spectrins: a structural platform for stabilization and activation of membrane channels, receptors and transporters.
Machnicka et al. (2014)
1 more

18 mentions without citation

Targeting the Plasmodium falciparum proteome and organelles for potential antimalarial drug candidates.
Abugri et al. (2022)
17 more