› 1s36

X-ray diffraction
1.96Å resolution

Crystal structure of a Ca2+-discharged photoprotein: Implications for the mechanisms of the calcium trigger and the bioluminescence

Released:
DOI: 10.2210/pdb1s36/pdb
PDB entry 1s36 coloured by chain, front view.
PDB entry 1s36 coloured by chain, side view.
PDB entry 1s36 coloured by chain, top view.
Source organism: Obelia longissima
Primary publication:
Crystal structure of a Ca2+-discharged photoprotein: implications for mechanisms of the calcium trigger and bioluminescence.
Deng L, Markova SV, Vysotski ES, Liu ZJ, Lee J, Rose J, Wang BC
J Biol Chem 279 33647-52 (2004)
PMID: 15155735

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173278 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Obelin Chain: A
Molecule details ›
Chain: A
Length: 195 amino acids
Theoretical weight: 22.22 KDa
Source organism: Obelia longissima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q27709 (Residues: 1-195; Coverage: 100%)
Sequence domains:
Structure domains: EF-hand

Ligands and Environments

4 bound ligands:
Ligand NA 1 x NA
Ligand CL 1 x CL
Ligand CEI 1 x CEI
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P41212
Unit cell:
a: 53.445Å b: 53.445Å c: 144.032Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.223 0.259
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Design and application of genetically encoded biosensors.
Palmer et al. (2011)
1 more

4 mentions without citation

Representation of Ion-Protein Interactions Using the Drude Polarizable Force-Field.
Li et al. (2015)
3 more