› 1s3i

X-ray diffraction
2.3Å resolution

Crystal structure of the N terminal hydrolase domain of 10-formyltetrahydrofolate dehydrogenase

Released:
DOI: 10.2210/pdb1s3i/pdb
PDB entry 1s3i coloured by chain, front view.
PDB entry 1s3i coloured by chain, side view.
PDB entry 1s3i coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
The crystal structure of the hydrolase domain of 10-formyltetrahydrofolate dehydrogenase: mechanism of hydrolysis and its interplay with the dehydrogenase domain.
Chumanevich AA, Krupenko SA, Davies C
J Biol Chem 279 14355-64 (2004)
PMID: 14729668

Function and Biology Details

Reaction catalysed: 
10-formyltetrahydrofolate + NADP(+) + H(2)O = tetrahydrofolate + CO(2) + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151161 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytosolic 10-formyltetrahydrofolate dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 310 amino acids
Theoretical weight: 34.08 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P28037 (Residues: 1-310; Coverage: 34%)
Gene names: Aldh1l1, Fthfd
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand BME 5 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21212
Unit cell:
a: 100Å b: 64.63Å c: 64.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.242 0.305
Expression system: Spodoptera frugiperda

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Citations

4 review citations

FDH: an aldehyde dehydrogenase fusion enzyme in folate metabolism.
Krupenko SA. (2009)
3 more

6 mentions without citation

Structural biology of the purine biosynthetic pathway.
Zhang et al. (2008)
5 more