› 1s3r

X-ray diffraction
2.6Å resolution

Crystal structure of the human-specific toxin intermedilysin

Released:
DOI: 10.2210/pdb1s3r/pdb
PDB entry 1s3r coloured by chain, front view.
PDB entry 1s3r coloured by chain, side view.
PDB entry 1s3r coloured by chain, top view.
Source organism: Streptococcus intermedius
Primary publication:
Insights into the action of the superfamily of cholesterol-dependent cytolysins from studies of intermedilysin.
Polekhina G, Giddings KS, Tweten RK, Parker MW
Proc Natl Acad Sci U S A 102 600-5 (2005)
PMID: 15637162

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-192252 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiol-activated cytolysin Chains: A, B
Molecule details ›
Chains: A, B
Length: 535 amino acids
Theoretical weight: 59.06 KDa
Source organism: Streptococcus intermedius
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9LCB8 (Residues: 34-532; Coverage: 100%)
Gene name: ily
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21212
Unit cell:
a: 88.45Å b: 173.64Å c: 105.11Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.265 0.229 0.295
Expression system: Escherichia coli

Quick links

Citations

23 review citations

Cholesterol-dependent cytolysins, a family of versatile pore-forming toxins.
Tweten RK. (2005)
22 more

19 mentions without citation

Membrane assembly of the cholesterol-dependent cytolysin pore complex.
Hotze et al. (2012)
18 more