› 1s4e

X-ray diffraction
2.9Å resolution

Pyrococcus furiosus galactokinase in complex with galactose, ADP and magnesium

Released:
DOI: 10.2210/pdb1s4e/pdb
PDB entry 1s4e coloured by chain, front view.
PDB entry 1s4e coloured by chain, side view.
PDB entry 1s4e coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Substrate specificity and mechanism from the structure of Pyrococcus furiosus galactokinase.
Hartley A, Glynn SE, Barynin V, Baker PJ, Sedelnikova SE, Verhees C, de Geus D, van der Oost J, Timson DJ, Reece RJ, Rice DW
J Mol Biol 337 387-98 (2004)
PMID: 15003454

Function and Biology Details

Reaction catalysed: 
ATP + alpha-D-galactose = ADP + alpha-D-galactose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190821 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Galactokinase Chains: A, B, C, D, E, F, G, H, I
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I
Length: 352 amino acids
Theoretical weight: 39.61 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HHB6 (Residues: 1-352; Coverage: 100%)
Gene names: PF0445, galK
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GLA 9 x GLA
Ligand MG 9 x MG
Ligand ADP 9 x ADP
1 modified residue:
Ligand MSE 36 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2221
Unit cell:
a: 211.974Å b: 355.672Å c: 165.613Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.23 0.27
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Enzymatic methods for glyco(diversification/randomization) of drugs and small molecules.
Gantt et al. (2011)
2 more

3 mentions without citation

The Gal3p transducer of the GAL regulon interacts with the Gal80p repressor in its ligand-induced closed conformation.
Lavy et al. (2012)
2 more