› 1s4u

X-ray diffraction
2.1Å resolution

Crystal Structure analysis of the beta-propeller protein Ski8p

Released:
DOI: 10.2210/pdb1s4u/pdb
PDB entry 1s4u coloured by chain, front view.
PDB entry 1s4u coloured by chain, side view.
PDB entry 1s4u coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of Ski8p, a WD-repeat protein with dual roles in mRNA metabolism and meiotic recombination.
Cheng Z, Liu Y, Wang C, Parker R, Song H
Protein Sci 13 2673-84 (2004)
PMID: 15340168

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169764 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Antiviral protein SKI8 Chain: X
Molecule details ›
Chain: X
Length: 407 amino acids
Theoretical weight: 45.22 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02793 (Residues: 1-397; Coverage: 100%)
Gene names: REC103, SKI8, YGL213C
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P212121
Unit cell:
a: 65.555Å b: 66.791Å c: 81.459Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.253 0.251 0.283
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Degradation of mRNAs that lack a stop codon: a decade of nonstop progress.
Klauer et al. (2012)
3 more

2 mentions without citation

Crystal structure of Ski8p, a WD-repeat protein with dual roles in mRNA metabolism and meiotic recombination.
Cheng et al. (2004)
1 more