› 1s6b

X-ray diffraction
1.6Å resolution

X-ray Crystal Structure of a Complex Formed Between Two Homologous Isoforms of Phospholipase A2 from Naja naja sagittifera: Principle of Molecular Association and Inactivation

Released:
DOI: 10.2210/pdb1s6b/pdb
PDB entry 1s6b coloured by chain, front view.
PDB entry 1s6b coloured by chain, side view.
PDB entry 1s6b coloured by chain, top view.
Source organism: Naja sagittifera
Primary publication:
Crystal structure of a calcium-induced dimer of two isoforms of cobra phospholipase A2 at 1.6 A resolution.
Jabeen T, Sharma S, Singh N, Singh RK, Kaur P, Perbandt M, Betzel Ch, Srinivasan A, Singh TP
Proteins 59 856-63 (2005)
PMID: 15828003

Function and Biology Details

Reaction catalysed: 
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157962 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Basic phospholipase A2 1 Chain: A
Molecule details ›
Chain: A
Length: 119 amino acids
Theoretical weight: 13.22 KDa
Source organism: Naja sagittifera
UniProt:
  • Canonical: P60043 (Residues: 8-126; Coverage: 94%)
Sequence domains: Phospholipase A2
Structure domains: Phospholipase A2 domain
Acidic phospholipase A2 2 Chain: B
Molecule details ›
Chain: B
Length: 119 amino acids
Theoretical weight: 13.29 KDa
Source organism: Naja sagittifera
UniProt:
  • Canonical: P60044 (Residues: 8-126; Coverage: 94%)
Sequence domains: Phospholipase A2
Structure domains: Phospholipase A2 domain

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand PO4 2 x PO4
Ligand ACY 2 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P41
Unit cell:
a: 64.537Å b: 64.537Å c: 57.117Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.206 0.236

Quick links

Citations

3 citation in other articles

Insights into the role of oligomeric state on the biological activities of crotoxin: crystal structure of a tetrameric phospholipase A2 formed by two isoforms of crotoxin B from Crotalus durissus terrificus venom.
Marchi-Salvador et al. (2008)
2 more

2 mentions without citation

Expression, functional, and structural analysis of proteins critical for otoconia development.
Xu et al. (2010)
1 more