› 1s6z

X-ray diffraction
1.5Å resolution

Enhanced Green Fluorescent Protein Containing the Y66L Substitution

Released:
DOI: 10.2210/pdb1s6z/pdb
PDB entry 1s6z coloured by chain, front view.
PDB entry 1s6z coloured by chain, side view.
PDB entry 1s6z coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
The crystal structure of the Y66L variant of green fluorescent protein supports a cyclization-oxidation-dehydration mechanism for chromophore maturation.
Rosenow MA, Huffman HA, Phail ME, Wachter RM
Biochemistry 43 4464-72 (2004)
PMID: 15078092

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.86 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
1 modified residue:
Ligand CR0 1 x CR0

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 50.8Å b: 62.739Å c: 69.966Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.189 0.223
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
9 more

3 mentions without citation

A peptide tag system for facile purification and single-molecule immobilization.
Huang et al. (2009)
2 more