› 1s81

X-ray diffraction
1.7Å resolution

PORCINE TRYPSIN WITH NO INHIBITOR BOUND

Released:
DOI: 10.2210/pdb1s81/pdb
PDB entry 1s81 coloured by chain, front view.
PDB entry 1s81 coloured by chain, side view.
PDB entry 1s81 coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
X-ray and NMR characterization of covalent complexes of trypsin, borate, and alcohols.
Transue TR, Krahn JM, Gabel SA, DeRose EF, London RE
Biochemistry 43 2829-39 (2004)
PMID: 15005618

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133375 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trypsin Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 23.49 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P00761 (Residues: 9-231; Coverage: 97%)
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

4 bound ligands:
Ligand CA 1 x CA
Ligand NA 1 x NA
Ligand SO4 1 x SO4
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 76.423Å b: 53.467Å c: 46.605Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.176 0.216

Quick links

Citations

2 review citations

In mesopore protein digestion: a new forthcoming strategy in proteomics.
Savino et al. (2011)
1 more

4 mentions without citation

Polyphenolic Compounds and Digestive Enzymes: In Vitro Non-Covalent Interactions.
Martinez-Gonzalez et al. (2017)
3 more