› 1s8g

X-ray diffraction
2.3Å resolution

Crystal structure of Lys49-Phospholipase A2 from Agkistrodon contortrix laticinctus, fatty acid bound form

Released:
DOI: 10.2210/pdb1s8g/pdb
PDB entry 1s8g coloured by chain, front view.
PDB entry 1s8g coloured by chain, side view.
PDB entry 1s8g coloured by chain, top view.
Source organism: Agkistrodon contortrix laticinctus
Primary publication:
A molecular mechanism for Lys49-phospholipase A2 activity based on ligand-induced conformational change.
Ambrosio AL, Nonato MC, de Araújo HS, Arni R, Ward RJ, Ownby CL, de Souza DH, Garratt RC
J Biol Chem 280 7326-35 (2005)
PMID: 15596433

Function and Biology Details

Reaction catalysed: 
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155863 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Basic phospholipase A2 homolog MT1 Chain: A
Molecule details ›
Chain: A
Length: 121 amino acids
Theoretical weight: 14.05 KDa
Source organism: Agkistrodon contortrix laticinctus
UniProt:
  • Canonical: P49121 (Residues: 17-137; Coverage: 100%)
Sequence domains: Phospholipase A2
Structure domains: Phospholipase A2 domain

Ligands and Environments

3 bound ligands:
Ligand SO4 1 x SO4
Ligand DAO 1 x DAO
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE D03B-MX1
Spacegroup: P41212
Unit cell:
a: 81.897Å b: 81.897Å c: 49.902Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.247

Quick links

Citations

4 review citations

Experimental pathology of local tissue damage induced by Bothrops asper snake venom.
Gutiérrez et al. (2009)
3 more

4 mentions without citation

Structural characterization of myotoxic ecarpholin S from Echis carinatus venom.
Zhou et al. (2008)
3 more