› 1sd0

X-ray diffraction
2.3Å resolution

Structure of arginine kinase C271A mutant

Released:
DOI: 10.2210/pdb1sd0/pdb
PDB entry 1sd0 coloured by chain, front view.
PDB entry 1sd0 coloured by chain, side view.
PDB entry 1sd0 coloured by chain, top view.
Source organism: Limulus polyphemus
Primary publication:
The active site cysteine of arginine kinase: structural and functional analysis of partially active mutants.
Gattis JL, Ruben E, Fenley MO, Ellington WR, Chapman MS
Biochemistry 43 8680-9 (2004)
PMID: 15236576

Function and Biology Details

Reaction catalysed: 
ATP + L-arginine = ADP + N(omega)-phospho-L-arginine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156357 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arginine kinase Chain: A
Molecule details ›
Chain: A
Length: 357 amino acids
Theoretical weight: 40.25 KDa
Source organism: Limulus polyphemus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P51541 (Residues: 1-357; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand NO3 1 x NO3
Ligand MG 1 x MG
Ligand CL 1 x CL
Ligand ARG 1 x ARG
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 64.905Å b: 71.255Å c: 79.98Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.209 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Relating structure to mechanism in creatine kinase.
McLeish et al. (2005)
2 more

1 mention without citation

Crystal structures of TM0549 and NE1324--two orthologs of E. coli AHAS isozyme III small regulatory subunit.
Petkowski et al. (2007)