› 1sd7

X-ray diffraction
2.65Å resolution

Crystal Structure of a SeMet derivative of MecI at 2.65 A

Released:
DOI: 10.2210/pdb1sd7/pdb
PDB entry 1sd7 coloured by chain, front view.
PDB entry 1sd7 coloured by chain, side view.
PDB entry 1sd7 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Crystal structures of the BlaI repressor from Staphylococcus aureus and its complex with DNA: insights into transcriptional regulation of the bla and mec operons.
Safo MK, Zhao Q, Ko TP, Musayev FN, Robinson H, Scarsdale N, Wang AH, Archer GL
J Bacteriol 187 1833-44 (2005)
PMID: 15716455

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methicillin resistance regulatory protein MecI Chains: A, B
Molecule details ›
Chains: A, B
Length: 123 amino acids
Theoretical weight: 15 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P68262 (Residues: 1-123; Coverage: 100%)
Gene name: mecI
Sequence domains: Penicillinase repressor
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P212121
Unit cell:
a: 67.61Å b: 70.174Å c: 75.009Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.254 0.241 0.317
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Coordination chemistry of bacterial metal transport and sensing.
Ma et al. (2009)
8 more

2 mentions without citation

Crystal structures of the BlaI repressor from Staphylococcus aureus and its complex with DNA: insights into transcriptional regulation of the bla and mec operons.
Safo et al. (2005)
1 more