› 1sdj

X-ray diffraction
2.3Å resolution

X-RAY STRUCTURE OF YDDE_ECOLI NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET ET25.

Released:
DOI: 10.2210/pdb1sdj/pdb
PDB entry 1sdj coloured by chain, front view.
PDB entry 1sdj coloured by chain, side view.
PDB entry 1sdj coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure and function of the phenazine biosynthetic protein PhzF from Pseudomonas fluorescens.
Blankenfeldt W, Kuzin AP, Skarina T, Korniyenko Y, Tong L, Bayer P, Janning P, Thomashow LS, Mavrodi DV
Proc Natl Acad Sci U S A 101 16431-6 (2004)
PMID: 15545603

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153478 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uncharacterized isomerase YddE Chain: A
Molecule details ›
Chain: A
Length: 310 amino acids
Theoretical weight: 34.07 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37757 (Residues: 1-297; Coverage: 100%)
Gene names: JW1459, b1464, yddE
Sequence domains: Phenazine biosynthesis-like protein
Structure domains: Diaminopimelate Epimerase; Chain A, domain 1

Ligands and Environments

1 bound ligand:
Ligand SO4 5 x SO4
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2221
Unit cell:
a: 125.047Å b: 130.659Å c: 48.555Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.26
Expression system: Escherichia coli BL21(DE3)

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Citations

11 review citations

Genomics of secondary metabolite production by Pseudomonas spp.
Gross et al. (2009)
10 more

2 mentions without citation

Crystal structure, catalytic mechanism, and mitogenic properties of Trypanosoma cruzi proline racemase.
Buschiazzo et al. (2006)
1 more