Function and Biology Details
Reaction catalysed:
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
Structure domain:
Structure analysis Details
Assembly composition:
homo dimer (preferred)
Assembly name:
BstYI (preferred)
PDBe Complex ID:
PDB-CPX-183013 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BstYI
Molecule details ›
Chain: A
Length: 203 amino acids
Theoretical weight: 23.22 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
Sequence domains: Restriction endonuclease BglII
Structure domains: Restriction Endonuclease
Length: 203 amino acids
Theoretical weight: 23.22 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
- Canonical:
Q84AF2 (Residues: 1-203; Coverage: 100%)
Sequence domains: Restriction endonuclease BglII
Structure domains: Restriction Endonuclease
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
APS BEAMLINE 19-ID
Spacegroup:
I222
Expression system: Escherichia coli
