› 1sf5

X-ray diffraction
1.1Å resolution

Structure of oxidized state of the P94A mutant of amicyanin

Released:
DOI: 10.2210/pdb1sf5/pdb
PDB entry 1sf5 coloured by chain, front view.
PDB entry 1sf5 coloured by chain, side view.
PDB entry 1sf5 coloured by chain, top view.
Source organism: Paracoccus denitrificans
Primary publication:
Structural studies of two mutants of amicyanin from Paracoccus denitrificans that stabilize the reduced state of the copper.
Carrell CJ, Sun D, Jiang S, Davidson VL, Mathews FS
Biochemistry 43 9372-80 (2004)
PMID: 15260480

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149611 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amicyanin Chain: A
Molecule details ›
Chain: A
Length: 105 amino acids
Theoretical weight: 11.5 KDa
Source organism: Paracoccus denitrificans
Expression system: Escherichia coli
UniProt:
  • Canonical: P22364 (Residues: 27-131; Coverage: 100%)
Gene names: ami, mauC
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand CU 1 x CU
1 modified residue:
Ligand MHO 1 x MHO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21
Unit cell:
a: 28.396Å b: 55.434Å c: 27.095Å
α: 90° β: 95.17° γ: 90°
R-values:
R R work R free
0.13 0.127 0.166
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Cupredoxins--a study of how proteins may evolve to use metals for bioenergetic processes.
Choi et al. (2011)
2 more