› 1sg5

Solution NMR

Solution structure of Yaeo, a Rho-specific inhibitor of transcription termination

Released:
DOI: 10.2210/pdb1sg5/pdb
PDB entry 1sg5 coloured by chain, ensemble of 20 models, front view.
PDB entry 1sg5 coloured by chain, ensemble of 20 models, side view.
PDB entry 1sg5 coloured by chain, ensemble of 20 models, top view.
Source organism: Escherichia coli
Primary publication:
Solution structure of YaeO, a Rho-specific inhibitor of transcription termination.
Gutiérrez P, Kozlov G, Gabrielli L, Elias D, Osborne MJ, Gallouzi IE, Gehring K
J Biol Chem 282 23348-53 (2007)
PMID: 17565995

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142758 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein rof Chain: A
Molecule details ›
Chain: A
Length: 86 amino acids
Theoretical weight: 9.71 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0AFW8 (Residues: 1-84; Coverage: 100%)
Gene names: JW0184, b0189, rof, yaeO
Sequence domains: Modulator of Rho-dependent transcription termination (ROF)
Structure domains: Rof-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

Termination and antitermination: RNA polymerase runs a stop sign.
Santangelo et al. (2011)
4 more

2 mentions without citation

Electrostatic Interactions Govern Extreme Nascent Protein Ejection Times from Ribosomes and Can Delay Ribosome Recycling.
Nissley et al. (2020)
1 more