› 1sgc

X-ray diffraction
1.8Å resolution

THE 1.8 ANGSTROMS STRUCTURE OF THE COMPLEX BETWEEN CHYMOSTATIN AND STREPTOMYCES GRISEUS PROTEASE A. A MODEL FOR SERINE PROTEASE CATALYTIC TETRAHEDRAL INTERMEDIATES

Released:
DOI: 10.2210/pdb1sgc/pdb
PDB entry 1sgc coloured by chain, front view.
PDB entry 1sgc coloured by chain, side view.
PDB entry 1sgc coloured by chain, top view.
Source organisms:
Primary publication:
The 1.8 A structure of the complex between chymostatin and Streptomyces griseus protease A. A model for serine protease catalytic tetrahedral intermediates.
Delbaere LT, Brayer GD
J Mol Biol 183 89-103 (1985)
PMID: 3892018

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins with specificity similar to chymotrypsin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133482 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Streptogrisin-A Chain: A
Molecule details ›
Chain: A
Length: 181 amino acids
Theoretical weight: 18.02 KDa
Source organism: Streptomyces griseus
Expression system: Not provided
UniProt:
  • Canonical: P00776 (Residues: 117-297; Coverage: 70%)
Gene name: sprA
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
CHYMOSTATIN A Chain: B
Molecule details ›
Chain: B
Length: 4 amino acids
Theoretical weight: 608 Da
Source organism: Streptomyces hygroscopicus
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PHA 1 x PHA

Experiments and Validation Details

Entry percentile scores
Spacegroup: P42
Unit cell:
a: 55.05Å b: 55.05Å c: 54.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.123 0.123 not available
Expression system: Not provided

Quick links

Citations

5 review citations

Cysteine proteases of positive strand RNA viruses and chymotrypsin-like serine proteases. A distinct protein superfamily with a common structural fold.
Gorbalenya et al. (1989)
4 more

4 mentions without citation

Recognition of functional sites in protein structures.
Shulman-Peleg et al. (2004)
3 more