› 1sh2

X-ray diffraction
2.3Å resolution

Crystal Structure of Norwalk Virus Polymerase (Metal-free, Centered Orthorhombic)

Released:
DOI: 10.2210/pdb1sh2/pdb
PDB entry 1sh2 coloured by chain, front view.
PDB entry 1sh2 coloured by chain, side view.
PDB entry 1sh2 coloured by chain, top view.
Source organism: Norwalk virus
Primary publication:
Crystal structure of norwalk virus polymerase reveals the carboxyl terminus in the active site cleft.
Ng KK, Pendás-Franco N, Rojo J, Boga JA, Machín A, Alonso JM, Parra F
J Biol Chem 279 16638-45 (2004)
PMID: 14764591

Function and Biology Details

Reaction catalysed: 
Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|- and the P1' position is occupied by Gly-|-
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180673 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA Polymerase Chain: A
Molecule details ›
Chain: A
Length: 510 amino acids
Theoretical weight: 56.81 KDa
Source organism: Norwalk virus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q70ET3 (Residues: 329-838; Coverage: 61%)
Sequence domains: Viral RNA-dependent RNA polymerase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: C2221
Unit cell:
a: 114.27Å b: 115.24Å c: 91.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.227 0.291
Expression system: Escherichia coli

Quick links

Citations

22 review citations

The epidemiologic and clinical importance of norovirus infection.
Atmar et al. (2006)
21 more

3 mentions without citation

Antiviral targets of human noroviruses.
Prasad et al. (2016)
2 more