› 1sh3

X-ray diffraction
2.95Å resolution

Crystal Structure of Norwalk Virus Polymerase (MgSO4 crystal form)

Released:
DOI: 10.2210/pdb1sh3/pdb
PDB entry 1sh3 coloured by chain, front view.
PDB entry 1sh3 coloured by chain, side view.
PDB entry 1sh3 coloured by chain, top view.
Source organism: Norwalk virus
Primary publication:
Crystal structure of norwalk virus polymerase reveals the carboxyl terminus in the active site cleft.
Ng KK, Pendás-Franco N, Rojo J, Boga JA, Machín A, Alonso JM, Parra F
J Biol Chem 279 16638-45 (2004)
PMID: 14764591

Function and Biology Details

Reaction catalysed: 
Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|- and the P1' position is occupied by Gly-|-
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180673 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA Polymerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 510 amino acids
Theoretical weight: 56.81 KDa
Source organism: Norwalk virus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q70ET3 (Residues: 329-838; Coverage: 61%)
Sequence domains: Viral RNA-dependent RNA polymerase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 105.05Å b: 109.14Å c: 112.044Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.215 0.28
Expression system: Escherichia coli

Quick links

Citations

24 review citations

The epidemiologic and clinical importance of norovirus infection.
Atmar et al. (2006)
23 more

6 mentions without citation

The structure of a birnavirus polymerase reveals a distinct active site topology.
Pan et al. (2007)
5 more