› 1sh5

X-ray diffraction
2Å resolution

Crystal structure of actin-binding domain of mouse plectin

Released:
DOI: 10.2210/pdb1sh5/pdb
PDB entry 1sh5 coloured by chain, front view.
PDB entry 1sh5 coloured by chain, side view.
PDB entry 1sh5 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Actin-binding domain of mouse plectin. Crystal structure and binding to vimentin.
Sevcík J, Urbániková L, Kost'an J, Janda L, Wiche G
Eur J Biochem 271 1873-84 (2004)
PMID: 15128297

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193044 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Plectin Chains: A, B
Molecule details ›
Chains: A, B
Length: 245 amino acids
Theoretical weight: 28.72 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9QXS1 (Residues: 180-417; Coverage: 5%)
Gene names: Plec, Plec1
Sequence domains: Calponin homology (CH) domain
Structure domains: Calponin-like domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X31
Spacegroup: P21
Unit cell:
a: 55.31Å b: 108.92Å c: 63.75Å
α: 90° β: 115.25° γ: 90°
R-values:
R R work R free
0.153 0.151 0.194
Expression system: Escherichia coli BL21

Quick links

Citations

14 review citations

Current insights into the formation and breakdown of hemidesmosomes.
Litjens et al. (2006)
13 more

1 mention without citation

Characterisation of Schizosaccharomyces pombe α-actinin.
Addario et al. (2016)