› 1si0

X-ray diffraction
1.35Å resolution

Crystal Structure of Mannheimia haemolytica Ferric iron-Binding Protein A in a closed conformation

Released:
DOI: 10.2210/pdb1si0/pdb
PDB entry 1si0 coloured by chain, front view.
PDB entry 1si0 coloured by chain, side view.
PDB entry 1si0 coloured by chain, top view.
Source organism: Mannheimia haemolytica
Primary publication:
Structural basis for iron binding and release by a novel class of periplasmic iron-binding proteins found in gram-negative pathogens.
Shouldice SR, Skene RJ, Dougan DR, Snell G, McRee DE, Schryvers AB, Tari LW
J Bacteriol 186 3903-10 (2004)
PMID: 15175304

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195573 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Periplasmic iron binding protein A FbpA Chain: A
Molecule details ›
Chain: A
Length: 320 amino acids
Theoretical weight: 35.89 KDa
Source organism: Mannheimia haemolytica
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Z4N6 (Residues: 23-342; Coverage: 100%)
Gene names: FEA53_08005, NCTC10638_03393, fbpA, futA1
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

3 bound ligands:
Ligand FE 1 x FE
Ligand CO3 1 x CO3
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: C2221
Unit cell:
a: 97.67Å b: 192.941Å c: 43.352Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.159 0.174
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Coordination chemistry of bacterial metal transport and sensing.
Ma et al. (2009)
6 more

4 mentions without citation

Photolysis of iron-siderophore chelates promotes bacterial-algal mutualism.
Amin et al. (2009)
3 more