› 1si7

X-ray diffraction
2.2Å resolution

Structure of E. coli tRNA psi 13 pseudouridine synthase TruD

Released:
DOI: 10.2210/pdb1si7/pdb
PDB entry 1si7 coloured by chain, front view.
PDB entry 1si7 coloured by chain, side view.
PDB entry 1si7 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of TruD, a novel pseudouridine synthase with a new protein fold.
Kaya Y, Del Campo M, Ofengand J, Malhotra A
J Biol Chem 279 18107-10 (2004)
PMID: 14999002

Function and Biology Details

Reaction catalysed: 
tRNA uridine(13) = tRNA pseudouridine(13)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176416 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA pseudouridine synthase D Chain: A
Molecule details ›
Chain: A
Length: 369 amino acids
Theoretical weight: 41.31 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q57261 (Residues: 1-349; Coverage: 100%)
Gene names: JW2715, b2745, truD, ygbO
Sequence domains: tRNA pseudouridine synthase D (TruD)
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P43
Unit cell:
a: 63.479Å b: 63.479Å c: 112.235Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.212 0.253
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Pseudouridine synthases.
Hamma et al. (2006)
6 more

3 mentions without citation

'Conserved hypothetical' proteins: prioritization of targets for experimental study.
Galperin et al. (2004)
2 more