› 1sj2

X-ray diffraction
2.41Å resolution

Crystal structure of Mycobacterium tuberculosis catalase-peroxidase

Released:
DOI: 10.2210/pdb1sj2/pdb
PDB entry 1sj2 coloured by chain, front view.
PDB entry 1sj2 coloured by chain, side view.
PDB entry 1sj2 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Crystal structure of Mycobacterium tuberculosis catalase-peroxidase.
Bertrand T, Eady NA, Jones JN, Nagy JM, Jamart-Grégoire B, Raven EL, Brown KA
J Biol Chem 279 38991-9 (2004)
PMID: 15231843

Function and Biology Details

Reaction catalysed: 
2 H(2)O(2) = O(2) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161625 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catalase-peroxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 743 amino acids
Theoretical weight: 81.06 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WIE5 (Residues: 2-740; Coverage: 100%)
Gene names: MTCY180.10, Rv1908c, katG
Sequence domains: Peroxidase
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
1 bound ligand:
Ligand GOL 6 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: P42212
Unit cell:
a: 150.33Å b: 150.33Å c: 154.281Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.211 0.268
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Why do bacteria use so many enzymes to scavenge hydrogen peroxide?
Mishra et al. (2012)
14 more

20 mentions without citation

Genomics, Computational Biology and Drug Discovery for Mycobacterial Infections: Fighting the Emergence of Resistance.
Munir et al. (2020)
19 more