› 1sj8

X-ray diffraction
2.6Å resolution

Solution Structure of the R1R2 Domains of Talin

Released:
DOI: 10.2210/pdb1sj8/pdb
PDB entry 1sj8 coloured by chain, front view.
PDB entry 1sj8 coloured by chain, side view.
PDB entry 1sj8 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Activation of a vinculin-binding site in the talin rod involves rearrangement of a five-helix bundle.
Papagrigoriou E, Gingras AR, Barsukov IL, Bate N, Fillingham IJ, Patel B, Frank R, Ziegler WH, Roberts GC, Critchley DR, Emsley J
EMBO J 23 2942-51 (2004)
PMID: 15272303

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150641 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Talin-1 Chain: A
Molecule details ›
Chain: A
Length: 308 amino acids
Theoretical weight: 31.51 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P26039 (Residues: 482-789; Coverage: 12%)
Gene names: Tln, Tln1
Sequence domains: Talin, middle domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: R32
Unit cell:
a: 105.81Å b: 105.81Å c: 173.792Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.26 0.258 0.297
Expression system: Escherichia coli BL21

Quick links

Citations

31 review citations

Focal adhesion kinase: in command and control of cell motility.
Mitra et al. (2005)
30 more

10 mentions without citation

How force might activate talin's vinculin binding sites: SMD reveals a structural mechanism.
Hytönen et al. (2008)
9 more