› 1sjw

X-ray diffraction
1.35Å resolution

Structure of polyketide cyclase SnoaL

Released:
DOI: 10.2210/pdb1sjw/pdb
PDB entry 1sjw coloured by chain, front view.
PDB entry 1sjw coloured by chain, side view.
PDB entry 1sjw coloured by chain, top view.
Source organism: Streptomyces nogalater
Primary publication:
Structure of the polyketide cyclase SnoaL reveals a novel mechanism for enzymatic aldol condensation.
Sultana A, Kallio P, Jansson A, Wang JS, Niemi J, Mäntsälä P, Schneider G
EMBO J 23 1911-21 (2004)
PMID: 15071504

Function and Biology Details

Reaction catalysed: 
Nogalaviketone = methyl nogalonate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193208 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nogalonic acid methyl ester cyclase Chain: A
Molecule details ›
Chain: A
Length: 144 amino acids
Theoretical weight: 16.79 KDa
Source organism: Streptomyces nogalater
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RN59 (Residues: 1-134; Coverage: 100%)
Gene name: snoaL
Sequence domains: SnoaL-like polyketide cyclase
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

1 bound ligand:
Ligand NGV 1 x NGV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: I222
Unit cell:
a: 69.1Å b: 72Å c: 65.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.143 0.143 0.176
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Type II polyketide synthases: gaining a deeper insight into enzymatic teamwork.
Hertweck et al. (2007)
7 more

8 mentions without citation

Rethinking Biosynthesis of Aclacinomycin A.
Xu et al. (2023)
7 more