› 1sjx

X-ray diffraction
2.2Å resolution

Three-Dimensional Structure of a Llama VHH Domain OE7 binding the cell wall protein Malf1

Released:
DOI: 10.2210/pdb1sjx/pdb
PDB entry 1sjx coloured by chain, front view.
PDB entry 1sjx coloured by chain, side view.
PDB entry 1sjx coloured by chain, top view.
Source organism: Lama glama
Primary publication:
Isolation of llama antibody fragments for prevention of dandruff by phage display in shampoo.
Dolk E, van der Vaart M, Lutje Hulsik D, Vriend G, de Haard H, Spinelli S, Cambillau C, Frenken L, Verrips T
Appl Environ Microbiol 71 442-50 (2005)
PMID: 15640220

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213092 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
immunoglobulin VH domain Chain: A
Molecule details ›
Chain: A
Length: 122 amino acids
Theoretical weight: 13.21 KDa
Source organism: Lama glama
Expression system: Saccharomyces cerevisiae
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand MPD 2 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P3221
Unit cell:
a: 71.4Å b: 71.4Å c: 74.84Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.191 0.206
Expression system: Saccharomyces cerevisiae

Quick links

Citations

26 review citations

Engineered antibody fragments and the rise of single domains.
Holliger et al. (2005)
25 more

7 mentions without citation

Crystal structure of the N-terminal domain of the secretin GspD from ETEC determined with the assistance of a nanobody.
Korotkov et al. (2009)
6 more