› 1skb

X-ray diffraction
1.58Å resolution

Crystallographic snapshots of Aspergillus fumigatus phytase revealing its enzymatic dynamics

Released:
DOI: 10.2210/pdb1skb/pdb
PDB entry 1skb coloured by chain, front view.
PDB entry 1skb coloured by chain, side view.
PDB entry 1skb coloured by chain, top view.
Source organism: Aspergillus fumigatus
Primary publication:
Crystallographic snapshots of Aspergillus fumigatus phytase, revealing its enzymatic dynamics.
Liu Q, Huang Q, Lei XG, Hao Q
Structure 12 1575-83 (2004)
PMID: 15341723

Function and Biology Details

Reaction catalysed: 
Myo-inositol hexakisphosphate + H(2)O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126114 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phytase A Chain: A
Molecule details ›
Chain: A
Length: 439 amino acids
Theoretical weight: 48.32 KDa
Source organism: Aspergillus fumigatus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: O00092 (Residues: 27-465; Coverage: 100%)
Gene names: AFUA_4G08630, phyA, phyA3
Sequence domains: Histidine phosphatase superfamily (branch 2)
Structure domains: Phosphoglycerate mutase-like

Ligands and Environments

1 bound ligand:
Ligand NAG 6 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P43212
Unit cell:
a: 70.131Å b: 70.131Å c: 186.237Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.155 0.182
Expression system: Komagataella pastoris

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Citations

1 review citation

Bioprocess for Production, Characteristics, and Biotechnological Applications of Fungal Phytases.
Jatuwong et al. (2020)
12 other articles

2 mentions without citation

The Sts Proteins: Modulators of Host Immunity.
Zaman et al. (2023)
1 more