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X-ray diffraction
3.2Å resolution

CRYSTAL STRUCTURE OF THE NUCLEOTIDE FREE ALPHA3BETA3 SUB-COMPLEX OF F1-ATPASE FROM THE THERMOPHILIC BACILLUS PS3

Released:
DOI: 10.2210/pdb1sky/pdb
PDB entry 1sky coloured by chain, front view.
PDB entry 1sky coloured by chain, side view.
PDB entry 1sky coloured by chain, top view.
Source organism: Bacillus sp. PS3
Primary publication:
The crystal structure of the nucleotide-free alpha 3 beta 3 subcomplex of F1-ATPase from the thermophilic Bacillus PS3 is a symmetric trimer.
Shirakihara Y, Leslie AG, Abrahams JP, Walker JE, Ueda T, Sekimoto Y, Kambara M, Saika K, Kagawa Y, Yoshida M
Structure 5 825-36 (1997)
PMID: 9261073

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139692 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ATP synthase subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 502 amino acids
Theoretical weight: 54.65 KDa
Source organism: Bacillus sp. PS3
Expression system: Escherichia coli
UniProt:
  • Canonical: P09219 (Residues: 1-502; Coverage: 100%)
Gene name: atpA
Sequence domains:
Structure domains:
ATP synthase subunit beta Chain: E
Molecule details ›
Chain: E
Length: 473 amino acids
Theoretical weight: 52 KDa
Source organism: Bacillus sp. PS3
Expression system: Escherichia coli
UniProt:
  • Canonical: P07677 (Residues: 1-473; Coverage: 100%)
Gene name: atpD
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: P213
Unit cell:
a: 159.5Å b: 159.5Å c: 159.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 0.299
Expression system: Escherichia coli

Quick links

Citations

38 review citations

The Mitochondrial Permeability Transition Pore: Channel Formation by F-ATP Synthase, Integration in Signal Transduction, and Role in Pathophysiology.
Bernardi et al. (2015)
37 more

12 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
11 more