› 1sl8

X-ray diffraction
1.7Å resolution

Calcium-loaded apo-aequorin from Aequorea victoria

Released:
DOI: 10.2210/pdb1sl8/pdb
PDB entry 1sl8 coloured by chain, front view.
PDB entry 1sl8 coloured by chain, side view.
PDB entry 1sl8 coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
All three Ca2+-binding loops of photoproteins bind calcium ions: the crystal structures of calcium-loaded apo-aequorin and apo-obelin.
Deng L, Vysotski ES, Markova SV, Liu ZJ, Lee J, Rose J, Wang BC
Protein Sci 14 663-75 (2005)
PMID: 15689515

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-139415 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aequorin-1 Chain: A
Molecule details ›
Chain: A
Length: 191 amino acids
Theoretical weight: 21.89 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P07164 (Residues: 6-196; Coverage: 97%)
Sequence domains:
Structure domains: EF-hand

Ligands and Environments

1 bound ligand:
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P43212
Unit cell:
a: 54.371Å b: 54.371Å c: 135.109Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.216 0.236
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Coelenterazine-dependent luciferases.
Markova et al. (2015)
3 more

2 mentions without citation

All three Ca2+-binding loops of photoproteins bind calcium ions: the crystal structures of calcium-loaded apo-aequorin and apo-obelin.
Deng et al. (2005)
1 more