› 1sli

X-ray diffraction
2Å resolution

LEECH INTRAMOLECULAR TRANS-SIALIDASE COMPLEXED WITH DANA

Released:
DOI: 10.2210/pdb1sli/pdb
PDB entry 1sli coloured by chain, front view.
PDB entry 1sli coloured by chain, side view.
PDB entry 1sli coloured by chain, top view.
Source organism: Macrobdella decora
Primary publication:
The crystal structure of an intramolecular trans-sialidase with a NeuAc alpha2-->3Gal specificity.
Luo Y, Li SC, Chou MY, Li YT, Luo M
Structure 6 521-30 (1998)
PMID: 9562562

Function and Biology Details

Reaction catalysed: 
Elimination of alpha-sialyl groups in N-acetylneuraminic acid glycosides, releasing 2,7-anhydro-alpha-N-acetylneuraminate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173277 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anhydrosialidase Chain: A
Molecule details ›
Chain: A
Length: 679 amino acids
Theoretical weight: 74.57 KDa
Source organism: Macrobdella decora
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q27701 (Residues: 81-759; Coverage: 92%)
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand DAN 1 x DAN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P1
Unit cell:
a: 46.443Å b: 69.516Å c: 72.514Å
α: 113.14° β: 95.39° γ: 106.87°
R-values:
R R work R free
0.167 0.167 0.224
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Lectins.
Vijayan et al. (1999)
2 more

4 mentions without citation

Unravelling the specificity and mechanism of sialic acid recognition by the gut symbiont Ruminococcus gnavus.
Owen et al. (2017)
3 more