› 1slj

Solution NMR

Solution structure of the S1 domain of RNase E from E. coli

Released:
DOI: 10.2210/pdb1slj/pdb
PDB entry 1slj coloured by chain, ensemble of 10 models, front view.
PDB entry 1slj coloured by chain, ensemble of 10 models, side view.
PDB entry 1slj coloured by chain, ensemble of 10 models, top view.
Source organism: Escherichia coli
Primary publication:
Structural characterization of the RNase E S1 domain and identification of its oligonucleotide-binding and dimerization interfaces.
Schubert M, Edge RE, Lario P, Cook MA, Strynadka NC, Mackie GA, McIntosh LP
J Mol Biol 341 37-54 (2004)
PMID: 15312761

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149268 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease E Chain: A
Molecule details ›
Chain: A
Length: 96 amino acids
Theoretical weight: 10.7 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P21513 (Residues: 35-125; Coverage: 9%)
Gene names: JW1071, ams, b1084, hmp1, rne
Sequence domains: S1 RNA binding domain
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli BL21

Quick links

Citations

6 review citations

RNA-binding proteins: modular design for efficient function.
Lunde et al. (2007)
5 more