› 1sqk

X-ray diffraction
2.5Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132189 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, alpha skeletal muscle Chain: A
Molecule details ›
Chain: A
Length: 377 amino acids
Theoretical weight: 42.1 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 1-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:
Ciboulot, isoform D Chain: B
Molecule details ›
Chain: B
Length: 25 amino acids
Theoretical weight: 2.79 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: O97428 (Residues: 10-34; Coverage: 19%)
Gene names: CG4944, Dmel\CG4944, Dmel_CG4944, EG0007-11, EG:EG0007.11, cib
Sequence domains: Thymosin beta-4 family

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand ADP 1 x ADP
Ligand LAR 1 x LAR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 67.494Å b: 75.198Å c: 85.314Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.278
Expression system: Escherichia coli

Quick links

Citations

33 review citations

The WASP-WAVE protein network: connecting the membrane to the cytoskeleton.
Takenawa et al. (2007)
32 more

11 mentions without citation

Actin structure and function.
Dominguez et al. (2011)
10 more