› 1sr8

X-ray diffraction
1.9Å resolution

Structural Genomics, 1.9A crystal structure of cobalamin biosynthesis protein (cbiD) from Archaeoglobus fulgidus

Released:
DOI: 10.2210/pdb1sr8/pdb
PDB entry 1sr8 coloured by chain, front view.
PDB entry 1sr8 coloured by chain, side view.
PDB entry 1sr8 coloured by chain, top view.
Source organism: Archaeoglobus fulgidus DSM 4304
Entry authors: Zhang R, Skarina T, Savchenko A, Edwards A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + cobalt-precorrin-5B = S-adenosyl-L-homocysteine + cobalt-precorrin-6A
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128141 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cobalt-precorrin-5B C(1)-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 298 amino acids
Theoretical weight: 33.22 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O29535 (Residues: 1-298; Coverage: 100%)
Gene names: AF_0723, cbiD
Sequence domains: CbiD
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: C2
Unit cell:
a: 79.035Å b: 75.912Å c: 63.032Å
α: 90° β: 113.7° γ: 90°
R-values:
R R work R free
0.209 0.209 0.258
Expression system: Escherichia coli BL21(DE3)

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