› 1ssc

X-ray diffraction
2Å resolution

THE 1.6 ANGSTROMS STRUCTURE OF A SEMISYNTHETIC RIBONUCLEASE CRYSTALLIZED FROM AQUEOUS ETHANOL. COMPARISON WITH CRYSTALS FROM SALT SOLUTIONS AND WITH RNASE A FROM AQUEOUS ALCOHOL SOLUTIONS

Released:
DOI: 10.2210/pdb1ssc/pdb
PDB entry 1ssc coloured by chain, front view.
PDB entry 1ssc coloured by chain, side view.
PDB entry 1ssc coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
1.6 A structure of semisynthetic ribonuclease crystallized from aqueous ethanol. Comparison with crystals from salt solutions and with ribonuclease A from aqueous alcohol solutions.
de Mel SJ, Doscher MS, Martin PD, Rodier F, Edwards BF
Acta Crystallogr D Biol Crystallogr 51 1003-12 (1995)
PMID: 15299768

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158391 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ribonuclease pancreatic Chain: A
Molecule details ›
Chain: A
Length: 112 amino acids
Theoretical weight: 12.38 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P61823 (Residues: 27-138; Coverage: 90%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain
Ribonuclease pancreatic Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.23 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P61823 (Residues: 140-150; Coverage: 9%)
Gene names: RNASE1, RNS1

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 30.36Å b: 38.34Å c: 53.55Å
α: 90° β: 106.35° γ: 90°
R-values:
R R work R free
0.166 not available not available
Expression system: Not provided

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Citations

1 citations in other articles

Predictive crystallization of ribonuclease A via rapid screening of osmotic second virial coefficients.
Tessier et al. (2003)

5 mentions without citation

Molecular evolution of B6 enzymes: binding of pyridoxal-5'-phosphate and Lys41Arg substitution turn ribonuclease A into a model B6 protoenzyme.
Vacca et al. (2008)
4 more