› 1sst

X-ray diffraction
2Å resolution

Serine Acetyltransferase- Complex with CoA

Released:
DOI: 10.2210/pdb1sst/pdb
PDB entry 1sst coloured by chain, front view.
PDB entry 1sst coloured by chain, side view.
PDB entry 1sst coloured by chain, top view.
Source organism: Haemophilus influenzae
Primary publication:
Structure of serine acetyltransferase in complexes with CoA and its cysteine feedback inhibitor.
Olsen LR, Huang B, Vetting MW, Roderick SL
Biochemistry 43 6013-9 (2004)
PMID: 15147185

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155144 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine acetyltransferase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 267 amino acids
Theoretical weight: 29.2 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P43886 (Residues: 1-267; Coverage: 100%)
Gene names: HI_0606, cysE
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand COA 3 x COA
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: C2221
Unit cell:
a: 111.3Å b: 125Å c: 103Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.223 0.27
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Selenium uptake, translocation, assimilation and metabolic fate in plants.
Sors et al. (2005)
11 more

3 mentions without citation

New insights into the structure and function of an emerging drug target CysE.
Verma et al. (2021)
2 more