› 1st2

X-ray diffraction
2Å resolution

THE THREE-DIMENSIONAL STRUCTURE OF BACILLUS AMYLOLIQUEFACIENS SUBTILISIN AT 1.8 ANGSTROMS AND AN ANALYSIS OF THE STRUCTURAL CONSEQUENCES OF PEROXIDE INACTIVATION

Released:
DOI: 10.2210/pdb1st2/pdb
PDB entry 1st2 coloured by chain, front view.
PDB entry 1st2 coloured by chain, side view.
PDB entry 1st2 coloured by chain, top view.
Source organism: Bacillus amyloliquefaciens
Primary publication:
The three-dimensional structure of Bacillus amyloliquefaciens subtilisin at 1.8 A and an analysis of the structural consequences of peroxide inactivation.
Bott R, Ultsch M, Kossiakoff A, Graycar T, Katz B, Power S
J Biol Chem 263 7895-906 (1988)
PMID: 3286644

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133530 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Subtilisin BPN' Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 27.6 KDa
Source organism: Bacillus amyloliquefaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00782 (Residues: 108-382; Coverage: 78%)
Gene name: apr
Sequence domains: Subtilase family
Structure domains: Peptidase S8/S53 domain

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand SO4 1 x SO4
1 modified residue:
Ligand MHO 3 x MHO

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 39.3Å b: 72.8Å c: 75.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.168 not available
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Subtilisin--an enzyme designed to be engineered.
Wells et al. (1988)
4 more

3 mentions without citation

Multiple structural alignment by secondary structures: algorithm and applications.
Dror et al. (2003)
2 more