› 1sto

X-ray diffraction
2.6Å resolution

CRYSTAL STRUCTURE OF OROTATE PHOSPHORIBOSYLTRANSFERASE

Released:
DOI: 10.2210/pdb1sto/pdb
PDB entry 1sto coloured by chain, front view.
PDB entry 1sto coloured by chain, side view.
PDB entry 1sto coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Primary publication:
Crystal structure of orotate phosphoribosyltransferase.
Scapin G, Grubmeyer C, Sacchettini JC
Biochemistry 33 1287-94 (1994)
PMID: 8312245

Function and Biology Details

Reaction catalysed: 
Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140401 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Orotate phosphoribosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 213 amino acids
Theoretical weight: 23.53 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Not provided
UniProt:
  • Canonical: P08870 (Residues: 1-213; Coverage: 100%)
Gene names: STM3733, pyrE
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand OH 1 x OH
Ligand OMP 1 x OMP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P41212
Unit cell:
a: 47.2Å b: 47.2Å c: 216.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.176 not available
Expression system: Not provided

Quick links

Citations

5 review citations

Structural analyses reveal two distinct families of nucleoside phosphorylases.
Pugmire et al. (2002)
4 more

6 mentions without citation

Assigning folds to the proteins encoded by the genome of Mycoplasma genitalium.
Fischer et al. (1997)
5 more