› 1svs

X-ray diffraction
1.5Å resolution

Structure of the K180P mutant of Gi alpha subunit bound to GppNHp.

Released:
DOI: 10.2210/pdb1svs/pdb
PDB entry 1svs coloured by chain, front view.
PDB entry 1svs coloured by chain, side view.
PDB entry 1svs coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Uncoupling conformational change from GTP hydrolysis in a heterotrimeric G protein alpha-subunit.
Thomas CJ, Du X, Li P, Wang Y, Ross EM, Sprang SR
Proc Natl Acad Sci U S A 101 7560-5 (2004)
PMID: 15128951

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145470 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 353 amino acids
Theoretical weight: 40.24 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P10824 (Residues: 2-354; Coverage: 100%)
Gene names: Gnai-1, Gnai1
Sequence domains: G-protein alpha subunit
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand GNP 1 x GNP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P3221
Unit cell:
a: 79.317Å b: 79.317Å c: 105.141Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.2 0.209
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Regulators of G-protein signaling and their Gα substrates: promises and challenges in their use as drug discovery targets.
Kimple et al. (2011)
2 more

4 mentions without citation

Structural comparison of unconventional G protein YchF with heterotrimeric G protein and small G protein.
Luo et al. (2022)
3 more