› 1sw1

X-ray diffraction
1.9Å resolution

Crystal structure of ProX from Archeoglobus fulgidus in complex with proline betaine

Released:
DOI: 10.2210/pdb1sw1/pdb
PDB entry 1sw1 coloured by chain, front view.
PDB entry 1sw1 coloured by chain, side view.
PDB entry 1sw1 coloured by chain, top view.
Source organism: Archaeoglobus fulgidus DSM 4304
Primary publication:
Structural basis for the binding of compatible solutes by ProX from the hyperthermophilic archaeon Archaeoglobus fulgidus.
Schiefner A, Holtmann G, Diederichs K, Welte W, Bremer E
J Biol Chem 279 48270-81 (2004)
PMID: 15308642

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128106 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ABC-type glycine betaine transport system substrate-binding domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 275 amino acids
Theoretical weight: 31.36 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli
UniProt:
  • Canonical: O29280 (Residues: 18-292; Coverage: 94%)
Gene name: AF_0982
Sequence domains: Substrate binding domain of ABC-type glycine betaine transport system
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 6 x ZN
Ligand PBE 2 x PBE
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 64.4Å b: 78.4Å c: 67.8Å
α: 90° β: 91.7° γ: 90°
R-values:
R R work R free
0.182 0.18 0.219
Expression system: Escherichia coli

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Citations

4 review citations

A structural classification of substrate-binding proteins.
Berntsson et al. (2010)
3 more

3 mentions without citation

AlphaFold2 protein structure prediction: Implications for drug discovery.
Borkakoti et al. (2023)
2 more