› 1sx6

X-ray diffraction
1.95Å resolution

Crystal structure of human Glycolipid Transfer protein in lactosylceramide-bound form

Released:
DOI: 10.2210/pdb1sx6/pdb
PDB entry 1sx6 coloured by chain, front view.
PDB entry 1sx6 coloured by chain, side view.
PDB entry 1sx6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for glycosphingolipid transfer specificity.
Malinina L, Malakhova ML, Teplov A, Brown RE, Patel DJ
Nature 430 1048-53 (2004)
PMID: 15329726

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192739 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Glycolipid transfer protein Chain: A
Molecule details ›
Chain: A
Length: 209 amino acids
Theoretical weight: 23.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NZD2 (Residues: 1-209; Coverage: 100%)
Gene name: GLTP
Sequence domains: Glycolipid transfer protein (GLTP)
Structure domains: Glycolipid transfer protein

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL
Carbohydrate polymer
3 bound ligands:
Ligand SPH 1 x SPH
Ligand OLA 1 x OLA
Ligand OCT 1 x OCT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: C2
Unit cell:
a: 75.599Å b: 49.073Å c: 68.538Å
α: 90° β: 122.52° γ: 90°
R-values:
R R work R free
0.195 0.192 0.244
Expression system: Escherichia coli

Quick links

Citations

25 review citations

Principles of lysosomal membrane digestion: stimulation of sphingolipid degradation by sphingolipid activator proteins and anionic lysosomal lipids.
Kolter et al. (2005)
24 more

12 mentions without citation

How α-Helical Motifs Form Functionally Diverse Lipid-Binding Compartments.
Malinina et al. (2017)
11 more