› 1sxh

X-ray diffraction
2.75Å resolution

apo structure of B. megaterium transcription regulator

Released:
DOI: 10.2210/pdb1sxh/pdb
PDB entry 1sxh coloured by chain, front view.
PDB entry 1sxh coloured by chain, side view.
PDB entry 1sxh coloured by chain, top view.
Source organism: Priestia megaterium
Primary publication:
Structural basis for allosteric control of the transcription regulator CcpA by the phosphoprotein HPr-Ser46-P.
Schumacher MA, Allen GS, Diel M, Seidel G, Hillen W, Brennan RG
Cell 118 731-41 (2004)
PMID: 15369672

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155515 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catabolite control protein A Chains: A, D
Molecule details ›
Chains: A, D
Length: 280 amino acids
Theoretical weight: 31.37 KDa
Source organism: Priestia megaterium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P46828 (Residues: 53-332; Coverage: 84%)
Gene name: ccpA
Sequence domains: Periplasmic binding protein-like domain
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 72.68Å b: 72.19Å c: 105.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.241 0.273
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

Carbon catabolite repression in bacteria: many ways to make the most out of nutrients.
Görke et al. (2008)
18 more

2 mentions without citation

Detection of translational noncrystallographic symmetry in Patterson functions.
Caballero et al. (2021)
1 more