› 1sxv

X-ray diffraction
1.3Å resolution

1.3A Crystal structure of rv3628, Mycobacterium tuberculosis inorganic pyrophosphatase (PPase) at pH5.0

Released:
DOI: 10.2210/pdb1sxv/pdb
PDB entry 1sxv coloured by chain, front view.
PDB entry 1sxv coloured by chain, side view.
PDB entry 1sxv coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Entry authors: Benini S, Wilson KS

Function and Biology Details

Reaction catalysed: 
Diphosphate + H(2)O = 2 phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161596 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inorganic pyrophosphatase Chain: A
Molecule details ›
Chain: A
Length: 172 amino acids
Theoretical weight: 19.54 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WI55 (Residues: 1-162; Coverage: 100%)
Gene names: MTCY15C10.24, Rv3628, ppa
Sequence domains: Inorganic pyrophosphatase
Structure domains: Inorganic pyrophosphatase

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P6322
Unit cell:
a: 98.46Å b: 98.46Å c: 97.353Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.155 0.154 0.169
Expression system: Escherichia coli BL21(DE3)

Quick links