› 1sy3

X-ray diffraction
1Å resolution

1.00 A Crystal Structure of D30N Mutant of Nitrophorin 4 from Rhodnius Prolixus Complexed with Nitric Oxide

Released:
DOI: 10.2210/pdb1sy3/pdb
PDB entry 1sy3 coloured by chain, front view.
PDB entry 1sy3 coloured by chain, side view.
PDB entry 1sy3 coloured by chain, top view.
Source organism: Rhodnius prolixus
Primary publication:
Role of binding site loops in controlling nitric oxide release: structure and kinetics of mutant forms of nitrophorin 4.
Maes EM, Weichsel A, Andersen JF, Shepley D, Montfort WR
Biochemistry 43 6679-90 (2004)
PMID: 15157102

Function and Biology Details

Reaction catalysed: 
3 nitrite + 2 H(+) = 2 nitric oxide + nitrate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188198 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrophorin-4 Chain: A
Molecule details ›
Chain: A
Length: 184 amino acids
Theoretical weight: 20.29 KDa
Source organism: Rhodnius prolixus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q94734 (Residues: 22-205; Coverage: 100%)
Sequence domains: Nitrophorin
Structure domains: Lipocalin

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
2 bound ligands:
Ligand PO4 1 x PO4
Ligand NO 1 x NO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: C2
Unit cell:
a: 70.431Å b: 42.729Å c: 53.124Å
α: 90° β: 94.27° γ: 90°
R-values:
R R work R free
0.143 0.142 0.159
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Iron transitions during activation of allosteric heme proteins in cell signaling.
Négrerie M. (2019)
38 other articles

3 mentions without citation

Hydration water and bulk water in proteins have distinct properties in radial distributions calculated from 105 atomic resolution crystal structures.
Chen et al. (2008)
2 more