› 1sz1

X-ray diffraction
6.21Å resolution

Mechanism of CCA-adding enzymes specificity revealed by crystal structures of ternary complexes

Released:
DOI: 10.2210/pdb1sz1/pdb
PDB entry 1sz1 coloured by chain, front view.
PDB entry 1sz1 coloured by chain, side view.
PDB entry 1sz1 coloured by chain, top view.
Source organisms:
Primary publication:
Mechanism of transfer RNA maturation by CCA-adding enzyme without using an oligonucleotide template.
Xiong Y, Steitz TA
Nature 430 640-5 (2004)
PMID: 15295590

Function and Biology Details

Reaction catalysed: 
(1a) a tRNA precursor + CTP = a tRNA with a 3' cytidine end + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127975 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
CCA-adding enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 437 amino acids
Theoretical weight: 51.47 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O28126 (Residues: 1-437; Coverage: 100%)
Gene names: AF_2156, cca
Sequence domains:
T-RNA (76-MER) Chains: E, F
Molecule details ›
Chains: E, F
Length: 76 nucleotides
Theoretical weight: 24.89 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
Sequence domains: tRNA

Ligands and Environments

No bound ligands
11 modified residues:
Ligand 2MG 2 x 2MG
Ligand H2U 4 x H2U
Ligand M2G 2 x M2G
Ligand OMC 2 x OMC
Ligand OMG 2 x OMG
Ligand YG 2 x YG
Ligand PSU 4 x PSU
Ligand 5MC 4 x 5MC
Ligand 7MG 2 x 7MG
Ligand 5MU 2 x 5MU
Ligand 1MA 2 x 1MA

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P321
Unit cell:
a: 218.403Å b: 218.403Å c: 170.263Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.361 0.36 0.38
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

21 review citations

tRNA nucleotidyltransferases: ancient catalysts with an unusual mechanism of polymerization.
Betat et al. (2010)
20 more

10 mentions without citation

RNA-specific ribonucleotidyl transferases.
Martin et al. (2007)
9 more