› 1sz9

X-ray diffraction
2.1Å resolution

The RNA polymerase II CTD in mRNA processing: beta-turn recognition and beta-spiral model

Released:
DOI: 10.2210/pdb1sz9/pdb
PDB entry 1sz9 coloured by chain, front view.
PDB entry 1sz9 coloured by chain, side view.
PDB entry 1sz9 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Recognition of RNA polymerase II carboxy-terminal domain by 3'-RNA-processing factors.
Meinhart A, Cramer P
Nature 430 223-6 (2004)
PMID: 15241417

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153821 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein PCF11 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 144 amino acids
Theoretical weight: 16.62 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P39081 (Residues: 1-140; Coverage: 22%)
Gene names: PCF11, YD9934.13C, YDR228C
Sequence domains: CID domain
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 56.73Å b: 67.67Å c: 135.637Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.22 0.252
Expression system: Escherichia coli

Quick links

Citations

49 review citations

Progression through the RNA polymerase II CTD cycle.
Buratowski S. (2009)
48 more

4 mentions without citation

Delineating the structural blueprint of the pre-mRNA 3'-end processing machinery.
Xiang et al. (2014)
3 more