› 1t02

X-ray diffraction
2.6Å resolution

Crystal structure of a Statin bound to class II HMG-CoA reductase

Released:
DOI: 10.2210/pdb1t02/pdb
PDB entry 1t02 coloured by chain, front view.
PDB entry 1t02 coloured by chain, side view.
PDB entry 1t02 coloured by chain, top view.
Source organism: Pseudomonas mevalonii
Primary publication:
Crystal structure of a statin bound to a class II hydroxymethylglutaryl-CoA reductase.
Tabernero L, Rodwell VW, Stauffacher CV
J Biol Chem 278 19933-8 (2003)
PMID: 12621048

Function and Biology Details

Reaction catalysed: 
(R)-mevalonate + CoA + 2 NAD(+) = 3-hydroxy-3-methylglutaryl-CoA + 2 NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-146717 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-hydroxy-3-methylglutaryl-coenzyme A reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 428 amino acids
Theoretical weight: 45.64 KDa
Source organism: Pseudomonas mevalonii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13702 (Residues: 1-428; Coverage: 100%)
Gene name: mvaA
Sequence domains: Hydroxymethylglutaryl-coenzyme A reductase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand LVA 1 x LVA
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: I4132
Unit cell:
a: 228.5Å b: 228.5Å c: 228.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.251
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Enzymes of the mevalonate pathway of isoprenoid biosynthesis.
Miziorko HM. (2011)
4 more

3 mentions without citation

An Atomic-Level Perspective of HMG-CoA-Reductase: The Target Enzyme to Treat Hypercholesterolemia.
Gesto et al. (2020)
2 more