› 1t0p

X-ray diffraction
1.66Å resolution

Structural Basis of ICAM recognition by integrin alpahLbeta2 revealed in the complex structure of binding domains of ICAM-3 and alphaLbeta2 at 1.65 A

Released:
DOI: 10.2210/pdb1t0p/pdb
PDB entry 1t0p coloured by chain, front view.
PDB entry 1t0p coloured by chain, side view.
PDB entry 1t0p coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An atomic resolution view of ICAM recognition in a complex between the binding domains of ICAM-3 and integrin alphaLbeta2.
Song G, Yang Y, Liu JH, Casasnovas JM, Shimaoka M, Springer TA, Wang JH
Proc Natl Acad Sci U S A 102 3366-71 (2005)
PMID: 15728350

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149032 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Integrin alpha-L Chain: A
Molecule details ›
Chain: A
Length: 175 amino acids
Theoretical weight: 19.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P20701 (Residues: 153-326; Coverage: 15%)
Gene names: CD11A, ITGAL
Sequence domains: von Willebrand factor type A domain
Structure domains: von Willebrand factor, type A domain
Intercellular adhesion molecule 3 Chain: B
Molecule details ›
Chain: B
Length: 86 amino acids
Theoretical weight: 9.03 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P32942 (Residues: 30-114; Coverage: 16%)
Gene name: ICAM3
Sequence domains: Intercellular adhesion molecule (ICAM), N-terminal domain
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 42.463Å b: 66.471Å c: 110.816Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.22 0.241
Expression systems:
  • Escherichia coli
  • Cricetulus griseus

Quick links

Citations

8 review citations

Rolling cell adhesion.
McEver et al. (2010)
7 more

26 mentions without citation

Structural basis of integrin regulation and signaling.
Luo et al. (2007)
25 more