› 1t24

X-ray diffraction
1.7Å resolution

Plasmodium falciparum lactate dehydrogenase complexed with NAD+ and 4-hydroxy-1,2,5-oxadiazole-3-carboxylic acid

Released:
DOI: 10.2210/pdb1t24/pdb
PDB entry 1t24 coloured by chain, front view.
PDB entry 1t24 coloured by chain, side view.
PDB entry 1t24 coloured by chain, top view.
Source organism: Plasmodium falciparum
Primary publication:
Identification and activity of a series of azole-based compounds with lactate dehydrogenase-directed anti-malarial activity.
Cameron A, Read J, Tranter R, Winter VJ, Sessions RB, Brady RL, Vivas L, Easton A, Kendrick H, Croft SL, Barros D, Lavandera JL, Martin JJ, Risco F, García-Ochoa S, Gamo FJ, Sanz L, Leon L, Ruiz JR, Gabarró R, Mallo A, Gómez de las Heras F
J Biol Chem 279 31429-39 (2004)
PMID: 15117937

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173280 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 322 amino acids
Theoretical weight: 34.98 KDa
Source organism: Plasmodium falciparum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q27743 (Residues: 1-316; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
2 bound ligands:
Ligand OXQ 1 x OXQ
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: I222
Unit cell:
a: 80.488Å b: 85.914Å c: 90.447Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.144 0.142 0.167
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Opportunities and challenges in antiparasitic drug discovery.
Pink et al. (2005)
12 more

3 mentions without citation

The Potential of Secondary Metabolites from Plants as Drugs or Leads against Protozoan Neglected Diseases-Part III: In-Silico Molecular Docking Investigations.
Ogungbe et al. (2016)
2 more