› 1t27

X-ray diffraction
2.2Å resolution

THE STRUCTURE OF PITP COMPLEXED TO PHOSPHATIDYLCHOLINE

Released:
DOI: 10.2210/pdb1t27/pdb
PDB entry 1t27 coloured by chain, front view.
PDB entry 1t27 coloured by chain, side view.
PDB entry 1t27 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structure of a multifunctional protein. Mammalian phosphatidylinositol transfer protein complexed with phosphatidylcholine.
Yoder MD, Thomas LM, Tremblay JM, Oliver RL, Yarbrough LR, Helmkamp GM
J Biol Chem 276 9246-52 (2001)
PMID: 11104777

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147728 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphatidylinositol transfer protein alpha isoform Chain: A
Molecule details ›
Chain: A
Length: 271 amino acids
Theoretical weight: 31.96 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P16446 (Residues: 1-271; Coverage: 100%)
Gene names: Pitpn, Pitpna
Sequence domains: Phosphatidylinositol transfer protein
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4

Ligands and Environments

1 bound ligand:
Ligand PCW 1 x PCW
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21
Unit cell:
a: 43.914Å b: 73.773Å c: 48.185Å
α: 90° β: 114.73° γ: 90°
R-values:
R R work R free
0.205 0.205 0.25
Expression system: Escherichia coli

Quick links

Citations

29 review citations

Organization and function of membrane contact sites.
Helle et al. (2013)
28 more

16 mentions without citation

Polyphosphoinositide-Binding Domains: Insights from Peripheral Membrane and Lipid-Transfer Proteins.
Pemberton et al. (2019)
15 more