› 1t2q

X-ray diffraction
1.83Å resolution

The Crystal Structure of an NNA7 Fab that recognizes an N-type blood group antigen

Released:
DOI: 10.2210/pdb1t2q/pdb
PDB entry 1t2q coloured by chain, front view.
PDB entry 1t2q coloured by chain, side view.
PDB entry 1t2q coloured by chain, top view.
Source organism: Mus musculus
Entry authors: Xie K, Song SC, Spitalnik SL, Wedekind JE

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106860 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ig-like domain-containing protein Chain: L
Molecule details ›
Chain: L
Length: 217 amino acids
Theoretical weight: 23.87 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: A2NHM3 (Residues: 1-217; Coverage: 99%)
Gene names: Igk-C, Igkc
Sequence domains:
Structure domains: Immunoglobulins
Ig heavy chain V region MC101 Chain: H
Molecule details ›
Chain: H
Length: 221 amino acids
Theoretical weight: 23.57 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01821 (Residues: 20-23, 24-116; Coverage: 100%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand GOL 2 x GOL
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 57.863Å b: 77.123Å c: 118.131Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.247
Expression system: Escherichia coli

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